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1.03915 Millipore

Triple sugar iron agar

for microbiology

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Properties

Related Categories Media by Application, Media for Cosmetic Industry, Microbiological Culture Media, Microbiology
Quality Level   100
form   medium granules (dehydrated (DCM))
mfr. no.   GRANUCULT®
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   7.4 (37 °C, 65 g/L in H2O, after autoclaving)
solubility   65 g/L
bulk density   650 kg/m3
Featured Industry   Food and Beverages
suitability   enterobacteriaceae
storage conditions   Store at +15°C to +25°C.

Description

Analysis Note

Appearance (clearness): clear
Appearance (colour): red
pH-value (25 °C): 7.2 - 7.6
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Citrobacter freundii ATCC 8090): good to very good
Growth (Enterobacter cloacae ATCC 13047 (WDCM 00083)): good to very good
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Proteus mirabilis ATCC 14153): good to very good
Growth (Proteus hauseri ATCC 13315): good to very good
Butt (Escherichia coli ATCC 25922 (WDCM 00013)): yellow
Butt (Citrobacter freundii ATCC 8090): yellow/black
Butt (Enterobacter cloacae ATCC 13047 (WDCM 00083)): yellow
Butt (Shigella flexneri ATCC 12022 (WDCM 00126)): yellow
Butt (Salmonella typhimurium ATCC 14028 (WDCM 00031)): yellow/black
Butt (Salmonella enteritidis ATCC 13076 (WDCM 00030)): yellow/black
Butt (Proteus mirabilis ATCC 14153): yellow/black
Butt (Proteus hauseri ATCC 13315): yellow/black
Slant surface (Escherichia coli ATCC 25922 (WDCM 00013)): yellow
Slant surface (Citrobacter freundii ATCC 8090): yellow
Slant surface (Enterobacter cloacae ATCC 13047 (WDCM 00083)): yellow
Slant surface (Shigella flexneri ATCC 12022 (WDCM 00126)): red
Slant surface (Salmonella typhimurium ATCC 14028 (WDCM 00031)): red
Slant surface (Salmonella enteritidis ATCC 13076 (WDCM 00030)): red
Slant surface (Proteus mirabilis ATCC 14153): red/black
Slant surface (Proteus hauseri ATCC 13315): yellow
Incubation: 24 hrs.; 35 °C; aerobic.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Cultivation of Shigella

The genus Shigella consists of four species: S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. These gram-negative, non-motile and non-sporulating bacilli belong to the Enterobacteriaceae family...
Keywords: AGE, Anaerobic, Culture media, Nucleic acid hybridization

ISO 6579-1 for Salmonella Detection in the Food Chain

The International Organization for Standardization (ISO) published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in the food product...
Keywords: Culture media, Environmental, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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