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1.04148 Millipore

Singlepath L′mono

for Listeria monocytogenes, input: food(s)

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Properties

Quality Level   100
shelf life   15 mo.
mfr. no.   SINGLEPATH®
application(s)   lateral flow assay: suitable
input   sample type food(s)
Featured Industry   Food and Beverages
storage temp.   2-8°C
suitability   Listeria monocytogenes
storage conditions   Store at +2°C to +8°C.

Description

General description

Rapid test for the detection of Listeria monocytogenes in foods

SINGLEPATH® L′mono is an immunological screening and an extremely fast confirmation test for Listeria monocytogenes based on immune flow principle. It is designed to avoid time-consuming and personnel intensive working steps for the application and interpretation of the tests. This is an immunochromatographic rapid test based on gold-labeled antibodies. This lateral flow test is a convenient and simple-to-use, one-step device. SINGLEPATH® L′mono test provides a clear presence/absence results within 20 minutes after sample enrichment. The lower detection limit can be regarded as 5×106 bacteria/ml or one colony on an agar plate, depending on serotype. SINGLEPATH® devices can be decontaminated by autoclave, bleach, etc. No additional equipment expenditure is needed. For optimized enrichment, our granulated culture media is recommended.

Application

SINGLEPATH® L′mono can be used in industrial microbiology, to test a wide range of foods for Listeria monocytogenes using Listeria selective agars (e.g. PALCAM, Oxford, Chromoplate Listeria, Chromocult Listeria, etc.). This test can also be used for confirmation of suspect Listeria monocytogenes colonies on these agars.

Linkage

Replaces: 1.04148.0002

Analysis Note

Performance test Antigen (Listeria monocytogenes Sv 1/2a EGD): passes test
Performance test Antigen (Listeria monocytogenes Sv 1/2c): passes test
Performance test Antigen (Listeria innocua ATCC 33090 (WDCM 00017)): passes test
Performance test Antigen (Listeria ivanovii ATCC 19119 (WDCM 00018)): passes test
Performance test Antigen (Escherichia coli ATCC 25922 (WDCM 00013)): passes test
Performance test Culture (Listeria monocytogenes Sv 1/2a EGD): passes test
Performance test Culture (Listeria innocua ATCC 33090 (WDCM 00017)): passes test
Performance test Culture (Listeria ivanovii ATCC 19119 (WDCM 00018)): passes test

Legal Information

SINGLEPATH is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Lateral Flow Tests for Immunological Detection in Food and Environmental Samples

Lateral flow tests are immunoassays for detecting food pathogens with the ultimate convenience. Covering the major pathogens, the tests act as mini-laboratories and include a built-in control reactio...
Keywords: Detection methods, Environmental, Immunoassay, Post translational modifications

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