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1.05287 Millipore

XLD (Xylose Lysine Deoxycholate) agar

acc. ISO 6579 GranuCult

Synonym: Xylose lysine deoxycholate agar

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Properties

Related Categories Media by Application, Media for Cosmetic Industry, Microbiological Culture Media, Microbiology
Quality Level   100
form   medium granules (dehydrated (DCM))
mfr. no.   GRANUCULT®
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   7.2-7.6 (25 °C, 55 g/L in H2O)
solubility   55 g/L
Featured Industry   Food and Beverages
suitability   Salmonella spp.
storage conditions   Store at +15°C to +25°C.

Description

General description

GranuCult is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Analysis Note

Appearance (clearness): clear to slightly opalescent
Appearance (colour): red
pH-value (25 °C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Colony count (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≥ 50 %
Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≥ 50 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): Growth or partial inhibition
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): Growth or partial inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): Colonies with black centre
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): Colonies with black centre
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): Yellow colonies
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): Yellow colonies
Incubation: 24 ± 3 hours at 37 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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ISO 6579-1 for Salmonella Detection in the Food Chain

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Salmonella Contamination and Testing

Foodborne illness affects public health and development worldwide. Salmonella spp. are members of the Enterobacteriaceae family and there are more than 2600 known serovars (or variations) within the ...
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