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1.05450 Millipore

Nutrient agar

acc. ISO 6579, ISO 10273 and ISO 21528 GranuCult

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Properties

Quality Level   100
form   medium granules (dehydrated (DCM))
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   7.0 (37 °C, 20 g/L in H2O, after autoclaving)
solubility   20 g/L
bulk density   600 kg/m3
Featured Industry   Food and Beverages
suitability   heterotrophic bacteria
  heterotrophic bacteria
storage conditions   Store at +15°C to +25°C.

Description

General description

GranuCult is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Analysis Note

Appearance (clearness): clear to slightly opalescent
Appearance (colour): yellowish-brown
pH-value (25 °C): 6.8 - 7.2
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Inoculum on reference medium (Yersinia enterocolitica ATCC 9610 (WDCM 00038)):
Inoculum on reference medium (Yersinia enterocolitica ATCC 23715 (WDCM 00160)):
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Colony count (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Colony count (Yersinia enterocolitica ATCC 9610 (WDCM 00038)):
Colony count (Yersinia enterocolitica ATCC 23715 (WDCM 00160)):
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 70 %
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≥ 70 %
Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≥ 70 %
Recovery on test medium (Yersinia enterocolitica ATCC 9610 (WDCM 00038)): ≥ 70 %
Recovery on test medium (Yersinia enterocolitica ATCC 23715 (WDCM 00160)): ≥ 70 %
Recovery (Staphylococcus aureus ATCC 25923 (WDCM00034)): ≥ 70 %
Incubation:
24 ± 2 at 37 ± 1 °C aerobic
Yersinia 24 ± 2 hours at 30 ± 1 °C aerobic
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Ask the Expert: How to Choose the Right Method for Microbial Testing in Infant Formula?

Infant formula and their ingredients are very sensitive foods which must be carefully microbiologically analyzed before release to market. Recent recalls of infant formula products due to microbial c...
Dr. Andreas Bubert
Senior Global Product Manager Culture Media F&B
Keywords: AGE, Culture media, Diseases, Environmental, Food & Beverage, Food Safety, Microbiology, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sample preparations, Separation

Culture Media and Tests for Beverage Testing

Spoilage organism testing is a crucial step within the beverage industry to enable the safe release of drinks to the market. Requirements can vary from industry to industry, depending on different sp...
Keywords: Culture media, Fermentation, Magnetic resonance spectroscopy

ISO 6579-1 for Salmonella Detection in the Food Chain

The International Organization for Standardization (ISO) published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in the food product...
Keywords: Culture media, Environmental, Microbiology

Revised EN ISO 21528:2017 for Detection and Enumeration of Enterobacteriaceae in the Food Chain

The International Organization for Standardization (ISO) published the revised EN ISO 21528-1 and EN ISO 21528-2 standards in 2017. In Part 1, a horizontal method for the detection of Enterobacteriac...
Keywords: Culture media, Environmental, Microbiology

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

The International Organization for Standardization (ISO), published revised EN ISO 11290-1 and EN ISO 11290-2 standards in 2017. In Part 1, a horizontal method for the detection of Listeria monocytog...
Keywords: Antibiotics, Culture media, Environmental, Microbiology

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