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1.05628 Supelco

HPTLC Silica gel 60 F₂₅₄

pkg of 25 plates, plate L × W 10 cm × 10 cm, glass support

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Properties

Related Categories Analytical/Chromatography, HPTLC Plates, LPLC/TLC/Paper Chromatography, Thin Layer Chromatography (TLC)
Quality Level   100
material   glass support
packaging   pkg of 25 plates
plate L × W   10 cm × 10 cm
storage conditions   no restrictions.

Description

General description

25 Glass plates 10 x 10 cm

HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

Application

Glass HPTLC Silica gel 60 F₂₅₄ plates. Silica gel HPTLC plates size 10 x 10 cm, 25 sheets.

Linkage

Replaces: 5628-5; 5628

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Detection and Determination of Caffeine, Taurine and Arginine in Shampoos by HPTLC

During the last few years a new trend in hair care products (shampoos) has become apparent. The cleaning ability of the product is no longer the primary focus as the cosmetic industry has started to ...
Keywords: Adsorption, Chromatography, Cosmetics, Derivatizations, Mass spectrometry, Nuclear magnetic resonance spectroscopy, Separation, Thin layer chromatography

Separation of Plant Pigments on Silica gel 60

From our library of Articles, Sigma-Aldrich presents Separation of Plant Pigments on Silica gel 60
Keywords: Sample preparations

Separation of plant pigments on Silica gel 60

From our library of Articles, Sigma-Aldrich presents Separation of plant pigments on Silica gel 60
Keywords: Sample preparations

TLC Analysis Ofacetylsalicylic Acid in Painkillers on HPTLC Silica gel 60 F254

From our library of Articles, Sigma-Aldrich presents TLC Analysis Ofacetylsalicylic Acid in Painkillers on HPTLC Silica gel 60 F254

TLC Analysis of Aflatoxin in walnut samples on HPTLC Silica gel 60 F254

From our library of Articles, Sigma-Aldrich presents TLC Analysis of Aflatoxin in walnut samples on HPTLC Silica gel 60 F254
Keywords: Thin layer chromatography

TLC Analysis of Lactose in different products on HPTLC Silica gel 60 F254

From our library of Articles, Sigma-Aldrich presents TLC Analysis of Lactose in different products on HPTLC Silica gel 60 F254
Keywords: Sample preparations, Thin layer chromatography

TLC Analysis of a cosmetic active on HPTLC Silica gel 60 F254 (analyte set 2)

From our library of Articles, Sigma-Aldrich presents TLC Analysis of a cosmetic active on HPTLC Silica gel 60 F254 (analyte set 2)
Keywords: Cosmetics, Sample preparations, Thin layer chromatography

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