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  • 1.05878 - MKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth (base)

1.05878 Millipore

MKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth (base)

according to ISO 6579, Granucult®



Agency/Method   according to ISO 6579
Quality Level   100
form   medium granules (dehydrated (DCM))
mfr. no.   Granucult®
application(s)   microbiological culture: suitable
  microbiology: suitable
Featured Industry   Food and Beverages
suitability   Salmonella spp.
storage conditions   Store at +15°C to +25°C.


General description

GranuCult is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

acc. ISO 6579 GranuCult

Analysis Note

Appearance (clearness): turbid with sediment
Appearance (colour): green
pH-value (25 °C): 7.8 - 8.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar

Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): > 10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): > 10 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): < 100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): < 100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): < 10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): < 10 colonies
MKTTn broth: 24 ± 3 hours at 37 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Ask the Expert: How to Choose the Right Method for Microbial Testing in Infant Formula?

Infant formula and their ingredients are very sensitive foods which must be carefully microbiologically analyzed before release to market. Recent recalls of infant formula products due to microbial c...
Dr. Andreas Bubert
Senior Global Product Manager Culture Media F&B
Keywords: AGE, Culture media, Diseases, Environmental, Food & Beverage, Food Safety, Microbiology, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sample preparations, Separation

ISO 6579-1 for Salmonella Detection in the Food Chain

The International Organization for Standardization (ISO) published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in the food product...
Keywords: Culture media, Environmental, Microbiology

Salmonella Contamination and Testing

Foodborne illness affects public health and development worldwide. Salmonella spp. are members of the Enterobacteriaceae family and there are more than 2600 known serovars (or variations) within the ...
Keywords: Diagnostic, Environmental, Genetic, Immunoassay, Immunodiffusion, Indicators, Ion-exchange chromatography, Microbiology, Phase transitions, Sample preparations, Separation

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