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1.06504 Millipore

Sodium deoxycholate

for microbiology

Synonym: Sodium deoxycholate

  • CAS Number 302-95-4

  • Empirical Formula (Hill Notation) C24H39NaO4

  • Molecular Weight 414.55

  •  MDL number MFCD00064139

  •  EC Index Number 206-132-7

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Properties

potency   1370 mg/kg LD50, oral (Rat)
application(s)   microbiology: suitable (preparation)
pH   7.5-9.0 (20 °C, 20 g/L in H2O)
mp   357-365 °C
transition temp   flash point >110 °C (own results)
solubility   330 g/L
bulk density   200 kg/m3
InChI   1S/C24H40O4.Na/c1-14(4-9-22(27)28)18-7-8-19-17-6-5-15-12-16(25)10-11-23(15,2)20(17)13-21(26)24(18,19)3;/h14-21,25-26H,4-13H2,1-3H3,(H,27,28);/q;+1/p-1/t14-,15-,16-,17+,18-,19+,20+,21+,23+,24-;/m1./s1
InChI key   FHHPUSMSKHSNKW-SMOYURAASA-M
storage conditions   Store at +15°C to +25°C.

Description

General description

Sodium deoxycholate is a water-soluble, bile salt and an ionic detergent used as a selective inhibitory agent for bacteriological culture media. It interferes with the growth and incorporation of glucose into various cellular fractions and also inhibits flagellum formation in gram negative bacteria.

Application

Sodium deoxycholate is used in number of bacteriological media for the isolation and classification of gram-negative bacteria from food and the environment.

Analysis Note

Assay (perchloric acid titration, calculated on dried substance): ≥ 98.5 %
Identity (IR-spectrum): passes test
pH-value (2 %; water): 7.5 - 9.5
Spec. rotation (α 20/D; 2 %; water; calc. on dried substance):
Heavy metals (as Pb): ≤ 0.001 %
TLC-Test: passes test
Cholic acid (TLC): ≤ 2.0 %
Loss on drying (TG): ≤ 6.0 %
Suitability for microbiology (MacConkey-Agar): passes test
The substance meets the criteria described in the monograph1483 Ph.Eur. 6th Ed. and any subsequently revised version.
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