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1.08487 Supelco

Urea

GR for analysis ACS,Reag. Ph Eur

Synonym: Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine, Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein/REAXYS Number 635724

  •  MDL number MFCD00008022

  •  EC Index Number 200-315-5

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Properties

Quality Level   200
grade   ACS reagent
  Reag. Ph. Eur.
vapor pressure   <0.1 hPa ( 20 °C)
potency   8471 mg/kg LD50, oral (Rat)
  8200 mg/kg LD50, skin (Rat)
pH   9 (20 °C, 100 g/L in H2O)
mp   132-135 °C (lit.)
density   1.335 g/mL at 25 °C (lit.)
bulk density   720‑760 kg/m3
SMILES string   NC(N)=O
InChI   1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
storage conditions   Store at +15°C to +30°C.

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Linkage

Replaces: UX0065-1

Analysis Note

Assay (ex N): 99.0 - 100.5 %
Assay (ex N, calc. on dried substance): 99.0 - 101.5 %
Purity (DSC (differential scanning calorimetry)): ≥ 99.5 %
Identity (IR-spectrum): passes test
Appearance of solution (10 %; Wasser; colour): colourless
Appearance of solution (10 %; Wasser; clarity): clear
In water insoluble matter: ≤ 0.003 %
Acidity, Alkalinity: ≤ 0.0005 meq/g
Melting point (DSC): 132 - 135 °C
NH₄ (Ammonium): ≤ 0.0500 %
Chloride (Cl): ≤ 0.0005 %
Sulfate (SO₄): ≤ 0.001 %
Heavy metals (as Pb): ≤ 0.0004 %
Biuret: ≤ 0.05 %
Sulfated ash (600 °C): ≤ 0.005 %
Cu (Copper): ≤ 0.0001 %
Fe (Iron): ≤ 0.0001 %
Pb (Lead): ≤ 0.0002 %
Loss on Drying (105°C): ≤ 1.0 %
Corresponds to ACS, Reag. Ph Eur

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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