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1.08492 Millipore

Urea agar (Base) acc. CHRISTENSEN

acc. ISO 6579, ISO 10273, ISO 19250, ISO 21567

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Properties

Quality Level   100
form   powder (dehydrated (DCM))
mfr. no.   GRANUCULT®
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   6.7-6.9 (37 °C, 21 g/L in H2O, after autoclaving)
solubility   21 g/L
bulk density   640 kg/m3
Featured Industry   Food and Beverages
suitability   urea-degrading bacteria
storage conditions   Store at +15°C to +25°C.

Description

Analysis Note

Appearance (clearness): clear
Appearance (color): red
pH-value (25 °C): 6.6 - 7.0
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Growth (Proteus mirabilis ATCC 29906 (WDCM 00023)): good to very good
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Growth (Yersinia enterocolitica ATCC 23715 (WDCM 00160)): good to very good
Growth (Yersinia enterocolitica DSM 13030 (WDCM 00216)): good to very good
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good
Growth (Escherichia coli DSM 19683 (WDCM 00179)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Shigella sonnei ATCC 29930 (WDCM 00127)): good to very good
Growth (Shigella flexneri ATCC 29903 (WDCM 00125)): good to very good
Colour change (Proteus mirabilis ATCC 29906 (WDCM 00023)): + (red/pink)
Colour change (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): + (red/pink)
Colour change (Yersinia enterocolitica ATCC 23715 (WDCM 00160)): + (red/pink)
Colour change (Yersinia enterocolitica DSM 13030 (WDCM 00216)): + (red/pink)
Colour change (Escherichia coli ATCC 8739 (WDCM 00012)): - or yellow
Colour change (Escherichia coli ATCC 25922 (WDCM 00013)): - or yellow
Colour change (Escherichia coli ATCC 11775 (WDCM 00090)): - or yellow
Colour change (Escherichia coli DSM 19683 (WDCM 00179)): - or yellow
Colour change (Salmonella typhimurium ATCC 14028 (WDCM 00031)): - or yellow
Colour change (Salmonella enteritidis ATCC 13076 (WDCM 00030)): - or yellow
Colour change (Shigella sonnei ATCC 29930 (WDCM 00127)): - or yellow
Colour change (Shigella flexneri ATCC 29903 (WDCM 00125)): - or yellow
Incubation: up to 24 hours at 37 ± 1 °C, Yersinia 24 ± 2 hours at 30 ± 1 °C, aerobic.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Ask the Expert: How to Choose the Right Method for Microbial Testing in Infant Formula?

Infant formula and their ingredients are very sensitive foods which must be carefully microbiologically analyzed before release to market. Recent recalls of infant formula products due to microbial c...
Dr. Andreas Bubert
Senior Global Product Manager Culture Media F&B
Keywords: AGE, Culture media, Diseases, Environmental, Food & Beverage, Food Safety, Microbiology, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sample preparations, Separation

Cultivation of Shigella

The genus Shigella consists of four species: S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. These gram-negative, non-motile and non-sporulating bacilli belong to the Enterobacteriaceae family...
Keywords: AGE, Anaerobic, Culture media, Nucleic acid hybridization

ISO 6579-1 for Salmonella Detection in the Food Chain

The International Organization for Standardization (ISO) published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in the food product...
Keywords: Culture media, Environmental, Microbiology

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