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1.09468 Sigma-Aldrich

Buffer tablets pH 7.2

for preparing buffer solution acc. to WEISE for staining of blood smears

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Properties

Related Categories Loading Buffer and Tracking Dyes, Molecular Biology, Nucleic Acid Electrophoresis More...
Quality Level   300
form   solid
IVD   for in vitro diagnostic use
application(s)   hematology: suitable
  histology: suitable
pH   7.2 (20 °C in H2O)
bulk density   1000 kg/m3
Featured Industry   Clinical Testing
Diagnostic Assay Manufacturing
storage conditions   Store at +5°C to +30°C.

Description

General description

Phosphate buffer solutions are used in hematology, for the dilution of staining solutions and for the rinsing steps in the staining process. The buffer tablets acc. to Weise will produce a buffered solution (pH6.4/pH6.8/pH7.2) that stabilizes the staining result and gives reproducible staining results. Buffer solution acc. to Weise pH 6.4 results in bright-orange stained erythrocytes and differentiated stained cells with nuclei, whereby the erythrocytes are stained orange when using pH 6.8. Buffer solution acc. to Weise pH 7.2 produces grayish stained erythrocytes and intense red-violet stained cells with nuclei. 100 tablets are sufficient for 100 l buffer solution, each produced solution can be stable up to 4 weeks. Buffer tablets acc. to Weise are IVD products and CE registered.

Analysis Note

pH-value (1 tablet in 1 l of water): 7.15 - 7.25
Crushing strength: ≥ 50 N
Disintegration time: ≤ 20 min
Suitability for blood-smear staining: conforms

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