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1.10398 Millipore

FRASER broth (base)

acc. ISO 11290 GranuCult

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Properties

Quality Level   100
form   medium granules (dehydrated (DCM))
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   7.0-7.4 (25 °C, 57.4 g/L in H2O)
solubility   57.4 g/L
Featured Industry   Food and Beverages
suitability   Listeria spp.
storage conditions   Store at +15°C to +25°C.

Description

Linkage

Replaces: 1.10398

Analysis Note

Appearance (clearness): clear to almost clear
Appearance (color): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Blacking (Listeria monocytogenes ATCC 13932 (WDCM 00021)): +
Blacking (Listeria monocytogenes ATCC 35152 (WDCM 00109)): +
Blacking (Escherichia coli ATCC 25922 (WDCM 00013)): -
Blacking (Escherichia coli ATCC 8739 (WDCM 00012)): -
Blacking (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Blacking (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Growth on ALOA medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): > 10 colonies
Growth on ALOA medium(Listeria monocytogenes ATCC 35152 (WDCM 00109)): > 10 colonies
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021)): blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109)): blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): < 100 colonien
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): < 100 colonies
Incubation:
Half-Fraser Broth: 24 ± 2 hours at 30 ± 1°C
Fraser Broth: 48 ± 2 hours at 37 ± 1°C
Confirmation on ALOA medium: 44 ± 4 hours at 37 ±1°C
Confirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°C

Legal Information

ALOA is a registered trademark of Biolife Italiana S.r.l.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Detection of Listeria monocytogenes in Food and Environmental Samples

20-minute quick learning: detection of Listeria monocytogenes according to international standards
Keywords: Safety industry

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

The International Organization for Standardization (ISO), published revised EN ISO 11290-1 and EN ISO 11290-2 standards in 2017. In Part 1, a horizontal method for the detection of Listeria monocytog...
Keywords: Antibiotics, Culture media, Environmental, Microbiology

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