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  • 1.11755 - PALCAM agar acc. VAN NETTEN et al. (Base)

1.11755 Millipore

PALCAM agar acc. VAN NETTEN et al. (Base)

acc. FDA-BAM and ref. to ISO 11290



Quality Level   100
form   powder (dehydrated (DCM))
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   6.9-7.1 (25 °C, 68.8 g/L in H2O, after autoclaving)
solubility   68.8 g/L
bulk density   650 kg/m3
Featured Industry   Food and Beverages
suitability   Listeria spp.
storage conditions   Store at +15°C to +25°C.


Analysis Note

Base tested with PALCAM-Listeria selective supplement acc. to VAN NETTEN et.al. art. no. 1.12122.
Appearance (clearness): clear to slightly opalescent
Appearance (color): red
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)):
Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 50 %
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 50 %
Recovery on test medium (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 50 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): partial inhibition
Black zone (Listeria monocytogenes ATCC 35152 (WDCM 00109)): black zone
Black zone (Listeria monocytogenes ATCC 13932 (WDCM 00021)): black zone
Black zone (Listeria innocua ATCC 33090 (WDCM 00017)): black zone
Incubation: up to 48 hours at 35 ± 1 °C, aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Detection of Listeria monocytogenes in Food and Environmental Samples

20-minute quick learning: detection of Listeria monocytogenes according to international standards
Keywords: Safety industry

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

The International Organization for Standardization (ISO), published revised EN ISO 11290-1 and EN ISO 11290-2 standards in 2017. In Part 1, a horizontal method for the detection of Listeria monocytog...
Keywords: Antibiotics, Culture media, Environmental, Microbiology

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