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1.15037 Supelco

HPTLC Silica gel 60 RP-18 with concentrating zone 20 x 2.5 cm

pkg of 25 plates, plate L × W 20 mm × 10 cm, according to DIN 38409-H13, glass support

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Properties

Related Categories Analytical/Chromatography, HPTLC Plates, LPLC/TLC/Paper Chromatography, Specialty Plates, Thin Layer Chromatography (TLC) More...
Quality Level   100
material   glass support
  silica gel 60 matrix
Agency/Method   according to DIN 38409-H13
analyte chemical class(es)   PAHs
packaging   pkg of 25 plates
layer thickness   250 μm
plate L × W   20 mm × 10 cm
storage conditions   no restrictions.

Description

General description

25 Glass plates 20 x 10 cm for PAH determination acc. to DIN 38409-H13

These plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples. Our concentrating zone plates are based on different adsorption properties of two adsorbents. The first is a large pore concentrating adsorbent where the samples are applied; the second is a selective layer for separation. Regardless of the spots’ shape, size, or position, the sample always concentrates as a narrow band where the two adsorbents overlap and where the separation starts. These plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples.

Linkage

Replaces: 15037-6; 15037

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
layer thickness concentrating zone: 70 - 110 µm
Fluctuation in the thickness of the concentration zone: ≤ 35 µm
Chromatographic testings:
A) cholesterin test
hRf-values
- cholesterin, cholesterin test:
11 - 23
B) Steroide test
- methyltestosterone, steroid test: 20 - 40
- Reichstein S, steroid test: 50 - 70
- hydrocortisone, steroid test: 65 - 85
Typical value determined on a activated plate (120°C, 15 min)
Eluent a) acetone/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)

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