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1.15936 Sigma-Aldrich

Erythrosine B (C.I. 45430)

for microscopy Certistain®

Synonym: Tetraiodofluorescein disodium salt, Erythrosine B (C.I. 45430)

  • CAS Number 16423-68-0

  • Empirical Formula (Hill Notation) C20H6I4Na2O5

  • Molecular Weight 879.86

  •  EC Index Number 240-474-8

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Indicator Solutions, Indicators, Titration More...
potency   1840 mg/kg LD50, oral (Rat)
pH   6-7 (20 °C, 10 g/L in H2O)
solubility   100 g/L
bulk density   470 kg/m3
storage conditions   Store at +5°C to +30°C.

Description

General description

Erythrosin B (C.I. 45430) is a CERTISTAIN dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Erythrosin B staining solutions.
As the dye belongs to the portfolio of Certistain dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed.

Analysis Note

Dye content (gravimetrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 524 - 527 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 930 - 1170
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Supplemental Hazard Statements 
harmful
WGK Germany 
2
RTECS 
LM5950000
Protocols & Articles
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