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1.15942 Sigma-Aldrich

Malachite green oxalate (C.I. 42000)

for microscopy Certistain®

Synonym: Malachite green oxalate (C.I. 42000)

  • CAS Number 2437-29-8

  • Empirical Formula (Hill Notation) C52H54N4O12

  • Molecular Weight 927.00

  •  EC Index Number 219-441-7

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Properties

Quality Level   400
form   solid
composition   Dye, ≥90% spectrophotometric assay
application(s)   hematology: suitable
  histology: suitable
pH   2.4 (24 °C, 10 g/L in H2O)
mp   159 °C
solubility   110 g/L
bulk density   400‑500 kg/m3
εmax   1730-1960 at 616-620 nm in water at 0.003 g/L
Featured Industry   Diagnostic Assay Manufacturing
InChI   1S/2C23H25N2.3C2H2O4/c2*1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3*3-1(4)2(5)6/h2*5-17H,1-4H3;3*(H,3,4)(H,5,6)/q2*+1;;;/p-2
InChI key   CNYGFPPAGUCRIC-UHFFFAOYSA-L
storage conditions   Store at +5°C to +30°C.

Description

General description

Malachite green oxalate (C.I. 42000) is a CERTISTAIN dry dye that is used for the preparation of a staining solution that is used for differential spore staining in bacteriological samples of human origin.
As CERTISTAIN dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.

Analysis Note

Dye content (spectrophotometrically): ≥ 90% (als C₄₆H₅₀N₄ *2 C₂HO₄ *C₂H₂O₄)
Identity
- oxalate:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 616 - 620 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; water): 1730 - 1960
TLC-Test: passes test
Loss on drying (50 °C / <1 hPa): ≤ 7%
Suitability for microscopy: passes test

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CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

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