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1.16141 Supelco

Sucrose (Saccharose) Test

Method: reflectometric with test strips and reagent 0.25 - 2.50 g/l Reflectoquant®

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Properties

packaging   50 tests in Plastic box (1.16141.0001)
storage conditions   Store at +2°C to +8°C.

Description

Analysis Note

function control: entspricht
Assay
- standard 1:
0.26 - 0.34 mg/l
- standard 2: 1.12 - 1.38 mg/l
- standard 3: 1.80 - 2.20 mg/l

General description

The Reflectoquant Sucrose test is suitable for determination of sucrose in beverages, e. g. fruit/vegetable juice and refreshment drinks after appropriate sample pretreatment or dilution. Sucrose (saccharose) is cleaved by sucrose phosphorylase into fructose and glucose-1-phosphate. The latter is converted by phosphoglucomutase into glucose-6-phosphate. This is in turn oxidized by NAD under the catalytic effect of glucose-6-phosphate dehydrogenase to gluconate-6-phosphate. In the presence of diaphorase the NADH formed in the process reduces a tetrazolium salt to a blue formazan that is determined reflectometrically on the RQflex. For the determination of only glucose please refer to our MQuant or Reflectoquant Glucose tests.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Application Notes Reflectometry – Sucrose (Saccharose) in Fruit Juices

This application note pertains to RQflex®20 and all discontinued instruments (RQflex® 10, RQflex® plus)
Keywords: Sample preparations

Application Notes Reflectometry – Sucrose (Saccharose) in Soft Drinks

This application note pertains to RQflex®20 and all discontinued instruments (RQflex® 10, RQflex® plus)
Keywords: High performance liquid chromatography, Sample preparations

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