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1.17952 Supelco

Hydroxymethylfurfural (HMF) Test

Method: reflectometric with test strips 1.0 - 60.0 mg/l Reflectoquant®



packaging   50 strips in Fibre case (1.17952.0001)
storage conditions   Store at +2°C to +8°C.


Analysis Note

function control: conforms

General description

The freshness of honey is determined by measuring its hydroxymethylfurfural (HMF) content. HMF is an organic compound that arises from the dehydration of fructose, e.g. when honey is heated for easier filling. HMF is barely detectable in freshly centrifuged honey, but increases at 2-3 mg/kg annually, depending on storage temperature and pH. At 21°C, HMF content can rise to 20 mg/kg in just one year. The Reflectoquant HMF Test is the first rapid test for the determination of HMF content, delivering accurate quantitative results in just a few minutes after sample preparation. Easy-to-use, portable and economical, the test is ideal for monitoring raw materials, as well as manufacturing and filling processes. This test provides a simple on-site alternative to time consuming HPLC analysis. On the test strip, HMF reacts with a barbituric acid derivative and an aminophenazone derivative to form a red-violet compound that is determined reflectometrically on the RQflex.

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Protocols & Articles


Application Notes Reflectometry – Hydroxymethylfurfural (HMF) in Elderberry Juice

This application note pertains to RQflex® 20 and all discontinued instruments (RQflex® 10, RQflex® plus)
Keywords: Sample preparations

Application Notes: Reflectometry – Hydroxymethylfurfural (HMF) in honey

Cat. No. 117952 Reflectoquant® Hydroxymethylfurfural (HMF) Test Cat. No. 117246 Reflectometer RQflex® 20 Cat. No. 116754 Water for analysis Cat. No. 116730 Reflectoquant® Blank Strips Cat. No. 117989...
Keywords: High performance liquid chromatography, Sample preparations

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