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12-370 Sigma-Aldrich

Normal Rabbit IgG

Normal Rabbit IgG Polyclonal Antibody control validated for use in Immunoprecipitation & Western Blotting.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibody Characterization and Analysis, Biochemicals and Reagents, Isotype Controls, Proteins and Derivatives
biological source   rabbit
clone   polyclonal
mfr. no.   Upstate®
concentration   1 mg/mL
application(s)   immunoprecipitation (IP): suitable
  western blot: suitable
isotype   IgG
conjugate   unconjugated

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

Research Category
Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Quality

Routinely evaluated by IP/WB as a non-specific IgG control.

Immunoprecipitation/Western Blot Assay:
2 µg of this lot was used as a non-specific IgG control in an immunoprecipitation / western blot assay.

Physical form

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide before the addition of glycerol to 30%. Liquid at –20°C.

Purified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product centrifuge the original vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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