• USA Home
  • 124011 - Akt Inhibitor IV - CAS 681281-88-9 - Calbiochem

EMAIL THIS PAGE TO A FRIEND
124011 Sigma-Aldrich

Akt Inhibitor IV - CAS 681281-88-9 - Calbiochem

The Akt Inhibitor IV, also referenced under CAS 681281-88-9, controls the biological activity of Akt. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

Synonym: Akt Inhibitor IV - CAS 681281-88-9 - Calbiochem

  • CAS Number 681281-88-9

  • Empirical Formula (Hill Notation) C31H27IN4S

  • Molecular Weight 614.54

  •  MDL number MFCD00570739

Purchase

Properties

Related Categories A to C, Akt, Biochemicals and Reagents, Enzyme Inhibitors, Enzyme Inhibitors by Enzyme,
Quality Level   100
assay   ≥98% (HPLC)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
  protect from light
storage conditions   +2C to +8C
color   yellow
solubility   DMSO: 5 mg/mL
shipped in   ambient

Description

General description

A cell permeable and reversible benzimidazole compound that inhibits Akt phosphorylation/activation by targeting the ATP binding site of a kinase upstream of Akt, but downstream of PI 3-K. Shown to block Akt-mediated FOXO1a nuclear export (IC50</sub> = 625 nM) and cell proliferation (IC50</sub><1.25 µM) in 786-O cells. Unlike phosphatidylinositol analog-based Akt inhibitors (Cat. Nos. 124005, 124008, 124009), this inhibitor does not affect PI3K. A 10 mM (1 mg/163 µl) solution of Akt Inhibitor IV (Cat. No. 124015) in DMSO is also available.

A cell-permeable and reversible benzimidazole compound that inhibits Akt phosphorylation/activation presumably by targeting the ATP binding site of a kinase upstream of Akt, but downstream of PI3-K. Shown to block Akt-mediated FOXO1a nuclear export (IC50</sub> = 625 nM) and cell proliferation (IC50</sub><1.25 µM) in 786-O cells. Unlike phosphatidylinositol analog-based Akt inhibitors (Cat. Nos. 124005, 124008, 124009), this inhibitor does not affect PI3-K.

Packaging

1 mg in Glass bottle

Packaged under inert gas

5 mg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Akt

Product does not compete with ATP.

Reversible: yes

Target IC50: 625 nM in blocking Akt-mediated FOXO1a nuclear export; < 1.25 µM in blocking Akt-mediated cell proliferation in 786-O cells

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Kau, T.R., et al. 2003. Cancer Cell4, 463.



Selected Citations
Lee, J., et al. 2009. Cell Stem Cell5, 76.


Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Cancer Stem Cell Detection Utilizing a Red-shifted Fluorescent Live Cell Probe for Aldehyde Dehydrogenase (ALDH), AldeRed™ 588-A.

Introduction Methods    Reagent Preparation    Stem Cell Sample Preparation    AldeRed ALDH Assay    Flow Cytometry Analysis Results Materials
Nick Asbrock1, Konstantin Taganov1, Vi Chu1, Martin G. Pomper2, Il Minn2, Haofan Wang2, Steven D. Leach2

1Assay & Platform Development, Bioscience BU, MilliporeSigma, 28820 Single Oak Drive, Temecula, CA, USA 9259
2Johns Hopkins Russel H. Morgan Department of Radiology and Radiological Science, Baltimore, Maryland 21287, USA.
Keywords: Cancer, Enzyme activity, Evaporation, Flow cytometry, Oxidations, Sample preparations

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?