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1.24823 Sigma-Aldrich

Nitro blue tetrazolium chloride (NBT)

for microscopy

Synonym: 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis [2-(4-nitrophenyl)-5-phe nyl-2H-tetrazolium chloride], NBT, Nitro blue tetrazolium chloride (NBT)

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Properties

form   solid
application(s)   hematology: suitable
  histology: suitable
mp   189 °C
εmax   800-900 at 256-259 nm in methanol at 0.01 g/L
Featured Industry   Diagnostic Assay Manufacturing
InChI   1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key   FSVCQIDHPKZJSO-UHFFFAOYSA-L
storage conditions   Store at +2°C to +8°C.

Description

General description

Nitro blue tetrazolium chloride (NBT) is a dry dye for the staining of histological tissue sections and histochemical as well as in vivo methods on material of human origin.
The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

for microscopy

Application

Dry dye used in histochemistry

Analysis Note

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (Methanol)256 - 259 nmSpec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; methanol; calc. on dried substance)800 - 900Loss on drying (Vacuum < 7 mbar, P₂O₅, 24 h)≤ 12 %Suitability for microscopypasses test

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