• USA Home
  • 128136 - 7α, 25-dihydroxycholesterol - Calbiochem

EMAIL THIS PAGE TO A FRIEND
128136 Sigma-Aldrich

7α, 25-dihydroxycholesterol - Calbiochem

Synonym: EBI2 agonist, EBI2 Activator, GPR183 Activator, 7alpha,25-OHC

  • Empirical Formula (Hill Notation) C27H46O3

  • Molecular Weight 418.65

Purchase

Properties

Related Categories Agonists, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, Endothelins,
feature productdata solubility   DMSO (25 mg/ml)
brand family   CBC
form   White powder
purity   ≥98% by HPLC
shipped in   1
storage conditions   21
  Y
  N

Description

General description

A highly potent and specific oxysterol EBI2 (GPR183) agonist with a kd of 450 pM in a saturation binding analysis, and activates EBI2 (EC50=140 pM compared with 2.1nM for its enantiomer 7b,25-OHC) in a GTP-γ assay. Dose-dependenty suppresses forskolin-induced cAMP accumulation in EBI2-expressing SK-N-MC/CRE-β galactosidase cell line with an IC50 of 2 nM, but not in control cells. Stimulates migration of LPS-activated spleen B cells and T cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells from EBI2-deficient mice.

Other Notes

Liu, C., et al. 2011. Nature475, 519.

Packaging

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

Warning

Standard Handling (A)

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?