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  • 14-383 - Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

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14-383 Sigma-Aldrich

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

  •  eCl@ss 32160405

  •  NACRES NA.84

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Properties

Related Categories Auxiliary Proteins, Biochemicals and Reagents, Other, Proteins and Derivatives
Quality Level   100
biological source   mouse
mfr. no.   Upstate®
application(s)   affinity binding assay: suitable
NCBI accession no.   NM_001015055.1
  NM_001015056.1
  NM_033046.2
UniProt accession no.   Q9BST9
shipped in   dry ice
Gene Information   mouse ... RTKN(20166)

Description

General description

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.

Product Source: Expressed in E. coli

Quality

Routinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778).

Physical form

650 µg of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 µg/mL aprotinin, 0.9 µg/mL leupeptin, and 10% glycerol.

Storage and Stability

Stable for 6 months at -70°C from date of receipt.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Peer-Reviewed Papers
15

References

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