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1.52026 Supelco

Chromolith® Flash NH2 25-4.6 HPLC column



Related Categories Analytical/Chromatography, HPLC/UHPLC More...
Quality Level   100
feature   endcapped: no
parameter   200 bar pressure
  45 °C max. temp.
application(s)   HPLC: suitable
  mass spectrometry (MS): suitable (Low - Medium)
matrix   highly porous
matrix active group   aminopropyl bonding phase
pore size   13 nm mesoporosity
  2 μm macroporosity
operating pH range   2 - 7.5
storage conditions   no restrictions.


General description

All Chromolith® NH2 columns are made of monolithic silica with a characteristic bimodal pore structure. Chromolith 4.6mm columns have macropores with 2 µm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is NH2.

Analysis Note

Pressure: ≤ 5 bar
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.35 %
Theoretical Plates (N/m) (Cytosine): ≥ 75000
Capacity factor (Cytosine): ≥ 1.0

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Chromolith® HPLC and UHPLC columns

Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology. There are no silica particles.
Keywords: High performance liquid chromatography, Sample preparations, Separation, Sol-gel

HPLC Analysis of B-Vitamins on Chromolith NH2 25 - 4.6mm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of B-Vitamins on Chromolith NH2 25 - 4.6mm
Keywords: High performance liquid chromatography

Monolithic Silica with Bimodal Pore Structure

Thanks to their patented monolithic silica technology, Chromolith® HPLC columns allow you to race through separations with maximum robustness and selectivity—at minimal back pressure.
Keywords: Filtration, High performance liquid chromatography, Precipitation, Sample preparations, Separation, Sol-gel, Solid phase extractions

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