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164598 Sigma-Aldrich

Aminopeptidase M, Porcine Kidney

Synonym: Aminopeptidase M, Porcine Kidney, Aminopeptidase M



Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
form   suspension (crystalline)
specific activity activity   ≥10 units/mg protein
  ≥50 units/mL
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
color   white
solubility   physiological buffer: soluble
  water: soluble
foreign activity   Oxidases ≤3%
shipped in   ambient


General description

Native amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.


50 u in Plastic ampoule

Please refer to vial label for lot-specific concentration.


Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.

Other Notes

Wachsmuth, E.E., et al. 1966. Biochemistry5, 169.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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