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171272 Sigma-Aldrich

AMPK Activator VI, RSVA314 - CAS 316130-82-2 - Calbiochem

The AMPK Activator VI, RSVA314 modulates the biological activity of AMPK. This small molecule/inhibitor is primarily used for Biochemicals applications.

Synonym: AMPK Activator VI, RSVA314 - CAS 316130-82-2 - Calbiochem, RSVA314, AMPK Signaling Activator VI

  • CAS Number 316130-82-2

  • Empirical Formula (Hill Notation) C18H21N3O3

  • Molecular Weight 327.38

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Properties

Related Categories AMP-Activated Protein Kinase (AMPK), Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Serine/Threonine Kinase Activators,
Quality Level   100
assay   >90% (HPLC)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  protect from light
storage conditions   -20C
color   yellow
solubility   DMSO: 50 mg/mL
shipped in   ambient

Description

General description

A cell permeable hydrozide that activates AMPK indirectly via a CaMKKβ-dependent pathway similar to resveratrol (Cat. No. 554325), which results in the phosphorylation of Thr-172 on the AMPKα2 subunit. This activator promotes lysosomal amyloid-β degradation in 3 cell lines nearly 40 times more potently than resveratrol with an IC50 of 1-2 µM. Treatment with RSVA314 at 1-3 µM results in a dose-dependent inhibition of the phosphorylation of 3 proteins downstream from mTOR activation including p70S6K, eukaryotic elongation factor 2 kinase (eEF2K), and S6 ribosomal protein, and promotes a robust increase in the phosphorylation of the protein raptor at Ser-792 in western blots. At the same concentrations, it elicits a strong, dose-dependent conversion of the light chain 3 (LC3) protein, a marker involved with autophagy induction, from LC3-I and LC3-II, also in western blots.

A cell permeable hydrozide that activates AMPK indirectly via a CaMKKβ-dependent pathway similar to resveratrol (Cat. No. 554325), which results in the phosphorylation of Thr-172 on the AMPKα2 subunit. This activator promotes lysosomal amyloid-β degradation in 3 cell lines nearly 40 times more potently than resveratrol with an IC50 of 1-2 µM. Treatment with RSVA314 at 1-3 µM results in a dose-dependent inhibition of the phosphorylation of 3 proteins downstream from mTOR activation including p70S6K, eukaryotic elongation factor 2 kinase (eEF2K), and S6 ribosomal protein, and promotes a robust increase in the phosphorylation of the protein raptor at Ser-792 in western blots. At the same concentrations, it elicits a strong, dose-dependent conversion of the light chain 3 (LC3) protein, a marker involved with autophagy induction, from LC3-I and LC3-II, also in western blots.

Packaging

10 mg in Glass bottle

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Other Notes

Vingtdeux, V., et al. 2011. FASEB J.25, 219.

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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