194434 Sigma-Aldrich

ABTS Chromophore

Synonym: ABTS Chromophore

  • Empirical Formula (Hill Notation) C18H18N4O6S4

  • Molecular Weight 514.62



Related Categories Biochemicals and Reagents, Colorimetric, Detection, Enzyme Substrates, Enzymes, Inhibitors, and Substrates More...
Quality Level   100
form   solution
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
color   clearvery pale yellow
shipped in   ambient


General description

Peroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.

Single component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.


100 ml in Glass bottle


Toxicity: Irritant (B)

Physical form

Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.


ABTS solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.

Other Notes

Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry39, 13201.

Legal Information

ABTS is a trademark of Boehringer Mannheim Corp.

ABTS is a trademark of Roche

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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