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203118 Sigma-Aldrich

Biotin-NHS, Water-Soluble

Synonym: Sulfobiotin-NHS, Sulfosuccinimidobiotin

  • Empirical Formula (Hill Notation) C14H18N3O8S2 · Na

  • Molecular Weight 443.43

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Properties

Related Categories Antibodies, Biotin/Avidin, Supplementary Products More...
color   off-white
form   solid
shipped in   ambient
solubility   DMSO: 10 mg/mL
  distilled water: 4 mg/mL
storage condition   OK to freeze
  desiccated
brand family   Calbiochem
purity   homogeneous single spot by TLC
storage conditions   +2C to +8C

Description

General description

Reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

Water-soluble form of biotin-NHS. Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.

Other Notes

Lisanti, M.P., et al. 1989. J. Cell Biol.109, 2145.

Packaging

50 mg in Plastic ampoule

Reconstitution

Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Warning

Standard Handling (A)

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
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