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203408-M Millipore

BLEOCIN Antibiotic, Streptomyces verticillus, Cell Culture-Tested - CAS 55658-47-4 - Calbiochem

suitable for cell culture

Synonym: BLEOCIN Antibiotic, Streptomyces verticillus, Cell Culture-Tested - CAS 55658-47-4 - Calbiochem

  • CAS Number 55658-47-4

  • Empirical Formula (Hill Notation) C57H89CuN19O21S2 · 3HCl

  • Molecular Weight 1613.49

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Properties

Quality Level   100
assay   ≥85% (HPLC)
form   lyophilized solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
  protect from light
storage conditions   +2C to +8C
application(s)   cell culture | mammalian: suitable
color   blue
solubility   water: 10 mg/mL
InChI   1S/C57H89N19O21S2.ClH/c1-22-35(73-48(76-46(22)61)27(14-33(60)80)68-15-26(59)47(62)86)52(90)75-37(43(28-16-65-21-69-28)95-56-45(41(84)39(82)31(17-77)94-56)96-55-42(85)44(97-57(63)92)40(83)32(18-78)93-55)53(91)70-24(3)38(81)23(2)49(87)74-36(25(4)79)51(89)67-13-8-34-71-30(20-98-34)54-72-29(19-99-54)50(88)66-12-7-11-64-10-6-5-9-58;/h16,19-21,23-27,31-32,36-45,55-56,64,68,77-79,81-85H,5-15,17-18,58-59H2,1-4H3,(H2,60,80)(H2,62,86)(H2,63,92)(H,65,69)(H,66,88)(H,67,89)(H,70,91)(H,74,87)(H,75,90)(H2,61,73,76);1H
InChI key   NRVKJXFKQWUKCB-UHFFFAOYSA-N

Description

General description

A unique antibiotic of the bleomycin family that is toxic to eukaryotic and prokaryotic cells. BLEOCIN Antibiotic cleaves double-stranded DNA and inhibits DNA synthesis, resulting in cell death. Can be used for selection of recombinant clones in a variety of cell types transfected with vectors containing a ble resistance marker. Copper content: ≥3.5%. Potency: ≥750 µg/mg.

A unique antibiotic of the bleomycin family that is toxic to eukaryotic and prokaryotic cells. BLEOCIN cleaves double-stranded DNA and inhibits DNA synthesis, resulting in cell death. Can be used for selection of recombinant clones in a variety of cell types transfected with vectors containing a ble resistance marker. Copper content: ≥3.5%. Potency: ≥750 µg/mg.

Warning

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Other Notes

Sergeev, D.S., et al. 1995. Bioorg. Khim. 21, 695.
Mulsant, P., et al. 1988. Somatic Cell and Molecular Genetics14, 243.
Ehrenfeld, G.M., et al. 1987. Biochemistry26, 931.
Takita, T., et al. 1972. J. Antibiot. 25, 755.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

RTECS 
EC5990700
Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

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