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208706 Sigma-Aldrich

Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda

Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 expressed in S. frugiperda insect cells.

Synonym: CaM Kinase II

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Properties

Quality Level   100
assay   ≥90% (SDS-PAGE)
form   liquid
specific activity   ≥250,000 units/mL
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   = -70C
foreign activity   Phosphatases, proteases, none detected
shipped in   wet ice

Description

General description

Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage.

Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. Reportedly involved in the modulation of neurite outgrowth by cAMP. Lower levels of CaM kinase II α-subunit predispose neurons to ischemic damage.

Packaging

5000 u in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.

Physical form

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween® 20 Detergent, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Tashima, K., et al. 1996. J. Neurochem. 66, 57.
Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
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