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208731 Sigma-Aldrich

Calpain Substrate II, Fluorogenic - CAS 94367-20-1 - Calbiochem

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2.

Synonym: Calpain Substrate II, Fluorogenic - CAS 94367-20-1 - Calbiochem, Suc-LY-AMC

  • CAS Number 94367-20-1

  • Empirical Formula (Hill Notation) C29H31N3O7

  • Molecular Weight 533.57

  •  MDL number MFCD00057900

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Properties

Quality Level   100
assay   ≥95% (TLC)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
  protect from light
storage conditions   -20C
color   off-white
solubility   DMSO: soluble
fluorescence   λex ~380  nm
  λem ~460 nm
shipped in   ambient
InChI   1S/C29H33N3O8/c1-4-16(2)27(32-24(34)11-12-25(35)36)29(39)31-22(14-18-5-8-20(33)9-6-18)28(38)30-19-7-10-21-17(3)13-26(37)40-23(21)15-19/h5-10,13,15-16,22,27,33H,4,11-12,14H2,1-3H3,(H,30,38)(H,31,39)(H,32,34)(H,35,36)/t16?,22?,27-/m0/s1
InChI key   VSWNAJAOJZXWBM-FTJGSZSVSA-N

Description

General description

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2. Also suitable for measuring the peptidase activity of the 20S proteasome. Typical concentrations range from 10 - 100 µM, depending on assay conditions.

Fluorogenic substrate for the quantitative determination of porcine calpain I and II activity. Also serves as a substrate for other proteases including proteasome. (excitation wavelength: 380 nm; emission wavelength: 460 nm)

Packaging

25 mg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: no

Primary Target
Calpain -1 and -2

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Suc-Leu-Tyr-AMC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Seol, J.H., et al. 1989. FEBS Lett. 247, 197.
Woo, K.M., et al. 1989. J. Biol. Chem. 264, 2088.
Sasaki, T., et al. 1984. J. Biol. Chem.259, 12489.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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