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208748 Sigma-Aldrich

Calpain-1 Substrate, Fluorogenic - Calbiochem

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

Synonym: μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH, Calpain-1 Substrate, Fluorogenic - Calbiochem

  • Empirical Formula (Hill Notation) C79H95N13O19S2

  • Molecular Weight 1594.81

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Properties

Quality Level   100
assay   ≥95% (HPLC)
form   lyophilized solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
  protect from light
storage conditions   -20C
color   red
solubility   DMSO: 1 mg/mL
fluorescence   λex ~490 nm
  λem ~518 nm
shipped in   wet ice

Description

General description

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence.

Packaging

2 mg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: no

Primary Target
A sensitive and specific substrate for calpain-1

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Mittoo, S., et al. 2003. Anal. Biochem.319, 234.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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