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208925 Sigma-Aldrich

Camptothecin, Camptotheca acuminata - CAS 2114454 - Calbiochem

A cell-permeable DNA topoisomerase I inhibitor.

Synonym: 4-Ethyl-4-hydroxy-1H-pyrano[3ʹ,4ʹ:6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)dione, Camptothecin, Camptotheca acuminata - CAS 2114454 - Calbiochem

  • CAS Number 7689-03-4

  • Empirical Formula (Hill Notation) C20H16N2O4

  • Molecular Weight 348.35

  •  MDL number MFCD00081076

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Properties

Related Categories Biochemicals and Reagents, Enzyme Inhibitors, Enzyme Inhibitors by Enzyme, Enzymes, Inhibitors, and Substrates, R to Z,
Quality Level   100
assay   ≥95% (HPLC)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
color   pale yellow
solubility   DMSO: 10 mg/mL
  methanol: 40 mg/mL
shipped in   ambient
InChI   1S/C20H16N2O4/c1-2-20(25)14-8-16-17-12(7-11-5-3-4-6-15(11)21-17)9-22(16)18(23)13(14)10-26-19(20)24/h3-8,25H,2,9-10H2,1H3/t20-/m0/s1
InChI key   VSJKWCGYPAHWDS-FQEVSTJZSA-N

Description

General description

A cell-permeable DNA topoisomerase I inhibitor. Exhibits anti-leukemic and antitumor properties. Induces apoptosis in HL-60 cells and mouse thymocytes. Arrests cells at the G2/M phase.

A cell-permeable, reversible DNA topoisomerase I inhibitor that binds to and stabilizes the topoisomerase-DNA covalent complex. Possesses antileukemic and antitumor properties. Inhibits Tat-mediated transactivation of HIV-1. Cytostatic for non-tumorigenic cells but cytotoxic for tumorigenic cells. Induces apoptosis of HL-60 cells and mouse thymocytes.

Packaging

50 mg in Alu drum

Biochem/physiol Actions

Cell permeable: yes

Primary Target
DNA topoisomerase 1

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

Preparation Note

Stock solutions made with methanol will remain cloudy.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Jones, C.B., et al. 1997. Cancer Chemother. Pharmacol.40, 475.
Staron, K., et al. 1994. Carcinogenesis 15, 2953.
Tanizawa, A., et al. 1994. J. Natl. Cancer Inst. 86, 836.
Gorczyca, W., et al. 1993. Toxicol. Lett.67, 249.
Onishi, Y., et al. 1993. Biochim. Biophys. Acta1175, 147.
Pantazis, P., et al. 1993. Int. J. Cancer53, 863.
Morham, S.G., and Shuman, S. 1992. J. Biol. Chem.267, 15984.
Hertzberg, R.P., et al. 1990. Biochem. J.28, 4629.
Hertzberg, R.P., et al. 1990. J. Biol. Chem.265, 19287.
Hsiang, Y.H., et al. 1985. J. Biol. Chem.260, 14873.

Safety & Documentation

Safety Information

RTECS 
UQ0492000
Protocols & Articles

Articles

Novel and Rapid Method to Assess Proliferative Properties of Mammalian Cells by Monitoring Cell Size Distributions

Much research has recently focused on elucidating the mechanisms by which eukaryotic cells regulate their size with respect to growth rate and cell cycle stage. These mechanisms are known to differ b...
Keywords: Apoptosis, Cell culture, Cell proliferation, Flow cytometry, High performance liquid chromatography, Sample preparations

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