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217356 Sigma-Aldrich

Carboxypeptidase B, Porcine Pancreas

Synonym: Carboxypeptidase B, Porcine Pancreas

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
form   lyophilized
specific activity activity   ≥100 units/mg protein
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C
pI    6.0
solubility   water: 1 mg/mL
foreign activity   Carboxypeptidase A <1.0%
  Chymotrypsin, none detected
  Trypsin ≤5%
shipped in   ambient

Description

General description

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn2+ chelators. Has an optimal pH of 7.0-9.0 and a pI of 6.0.

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn2+ chelators.

Packaging

1 mg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Hip-Arg per min at 25°C, pH 7.7.

Physical form

Lyophilized from 360 µM HEPES, 1.41 µM zinc chloride, 1.41 mg/ml Trehalose, pH 7.4.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Folk, J.E. 1970. Methods Enzymol.19, 504.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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