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217605 Sigma-Aldrich

Anti-CD54 Mouse mAb (15.2)

liquid, ≥95% (SDS-PAGE), clone 15.2

Synonym: Anti-ICAM-1, Domain 1

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Properties

Related Categories Alphabetical Index, Antibodies, CD0-CDC, Primary Antibodies
clone   15.2, monoclonal
biological source   mouse
species reactivity   human
form   liquid
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
assay   ≥95% (SDS-PAGE)
contains   0.5 mg/mL gentamicin sulfate as preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C

Description

General description

Anti-CD54, mouse monoclonal, clone 15.2, recognizes D1 domain of the ~90 kDa CD54 (ICAM-1) in Raji cells. It is validated for FC, WB, IP, IHC on frozen sections, and neutralization studies.

Protein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).

Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.

Immunogen

Human

human monocytes

Application

Flow Cytometry (1-10 µg/ml)

Frozen Sections (see comments)

Immunoblotting (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

100 μg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Raji cells

Other Notes

FACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell68, 71.
Dransfield, I., et al. 1992. J. Cell Biol.116, 1527.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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