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218765 Sigma-Aldrich

Caspase-9 Substrate I, Fluorogenic - Calbiochem

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6).

Synonym: Ac-LEHD-AFC, Caspase-9 Substrate I, Fluorogenic - Calbiochem

  • Empirical Formula (Hill Notation) C33H38F3N7O11

  • Molecular Weight 765.69

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Properties

Related Categories Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Protease, Substrates by Enzyme More...
Quality Level   100
assay   ≥95% (HPLC)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
  protect from light
storage conditions   -20C
color   white
solubility   DMSO: 5 mg/mL
fluorescence   λex ~400  nm
  λem ~505 nm
shipped in   ambient

Description

General description

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Packaging

1 mg in Glass bottle

5 mg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: no

Primary Target
A fluorogenic substrate for caspase-9

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Leu-Glu-His-Asp-AFC

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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