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218813 Sigma-Aldrich

Caspase-2, Human, Recombinant, E. coli

Caspase-2, Human, Recombinant, E. coli is expressed as a single polypeptide chain that undergoes spontaneous autoprocessing to yield the native active enzyme.

Synonym: ICH-1, Nedd-2

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
assay   ≥90% (SDS-PAGE)
form   lyophilized solid
specific activity   ≥1500 units/mg protein
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   = -70C
solubility   PBS: 0.1-1 units/μL
shipped in   wet ice

Description

General description

Recombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.

Packaging

100 u in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-pNA (Cat. No. 218820) per h at 37°C, pH 7.2.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.

Other Notes

Talanian, R.V., et al. 1997. J. Biol. Chem.272, 9677.
Wang, L., et al. 1994. Cell78, 739.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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