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228180 Sigma-Aldrich

Cholesterol Esterase, Microbial

Cholesterol Esterase, Microbial, CAS 9026-00-0, is a native enzyme that catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

Synonym: CE, Sterolester Acylhydrolase, Cholesterol Esterase, Microbial

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
form   powder
specific activity activity   ≥100 units/mg protein
  ≥70 units/mg dry wt.
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
  protect from light
storage conditions   -20C
color   light brown
solubility   10 mM potassium phosphate/sodium hydroxide, 0.1% BSA, pH 7.5: soluble
foreign activity   Catalase ≤1.5%
shipped in   ambient

Description

General description

Native, microbial cholesterol esterase. Catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

Packaging

1000 u in Plastic ampoule

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol cholesterol ester per min at 37°C, pH 6.5.

Reconstitution

Reconstitute just prior to use. Enzyme will lose activity in solution upon storage.

Other Notes

Allain, C.C., et al. 1974. Clin. Chem.20, 470.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
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