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  • 23-044-M - VHL Protein Complex, Active, 10 µg

23-044-M Sigma-Aldrich

VHL Protein Complex, Active, 10 µg

Active complex of five (5) recombinant human enzymes: VHL amino acids 54-end, full length Elongin C, full length Elongin B, full length Cul2, & full length Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

Synonym: VCB-Cul2 complex, ECV complex, CBC VHL

  •  eCl@ss 32160405

  •  NACRES NA.41



Related Categories Application Index, Biochemicals and Reagents, Cell Signaling Enzymes, Enzymes, Inhibitors, and Substrates, Enzymes/Proteins,
Quality Level   100
biological source   human
mfr. no.   Upstate®
application(s)   activity assay: suitable
NCBI accession no.   NM_000551
  NM_005648 (Elongin C)
  NM_007108 (Elongin B)
Gene Information   human ... Cul2(8453), Rbx1(9978), VHL(7428)


General description

The VHL complex is a multi-subunit ubiquitin ligase composed of VHL, Elongin B, Elongin C, Cul2 and Rbx1. The VHL protein serves as the substrate recognition component and is linked by Elongin C to a heterodimeric Cul2/Rbx1 module that functions as a potent activator of the ubiquitination of target proteins by an E2 conjugating enzyme. Elongin B interacts with the complex through Elongin C and appears to stabilize the binding of Elongin C to VHL. The primary function of the VHL complex is to regulate HIF (hypoxia inducible factor) activity by targeting the hydroxylated HIF-1α subunit for ubiquitination and rapid proteasomal degradation under normoxic conditions. It therefore plays an important role in the regulation of hypoxia-inducible genes such as the vascular endothelial growth factor (VEGF) and glucose transport-1 (Glut-1). Mutations in VHL are associated with the inherited von Hippel-Lindau (VHL) cancer syndrome and numerous forms of renal cell carcinoma.


Ubiquitination Cascade Comoponent: E3

Physical form

Purified using glutathione sepharose.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


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