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233156 Millipore

Cleland’s Reagent, Molecular Biology Grade - CAS 3483-12-3 - Calbiochem

A protective agent for SH groups.

Synonym: 1,4-Dithiothreitol, DTT, Cleland’s Reagent, Molecular Biology Grade - CAS 3483-12-3 - Calbiochem

  • CAS Number 3483-12-3

  • Empirical Formula (Hill Notation) C4H10O2S2

  • Molecular Weight 154.25

  •  MDL number MFCD00004877

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Properties

Related Categories Molecular Biology, Protein Electrophoresis, Proteomics, Reducing Agents, SDS-polyacrylamide gel electrophoresis (PAGE) More...
Quality Level   100
assay   ≥99% (titration)
form   powder
  solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
color   white
solubility   water: 15 mg/mL
cation traces   heavy metals: ≤0.001%
absorption   ≤0.100 at 280 nm at 100 mM (1 cm)
  ≤0.500 at 260 nm at 100 mM (1 cm)
foreign activity   DNases, proteases, RNases, none detected
shipped in   ambient
InChI   1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
InChI key   VHJLVAABSRFDPM-IMJSIDKUSA-N

Description

General description

A protective agent for SH groups.

Water-soluble protective reagent for sulfhydryl groups [absorbance (100 mM): ≤0.5 at 260 nm; ≤0.1 at 280 nm]. It is capable of maintaining monothiols completely in the reduced state and of reducing disulfides quantitatively. Since it readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation.

Packaging

1, 10 g in Plastic ampoule

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Protocols & Articles
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