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234134 Sigma-Aldrich

Collagenase, Type III, Clostridium histolyticum

Synonym: Collagenase, Type III, Clostridium histolyticum

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
form   lyophilized
specific activity activity   ≥2000 BTC units/mg protein
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
storage conditions   -20C
foreign activity   Non-specific proteolytic ≤5.0 fluorescence units/mg
shipped in   ambient

Description

General description

This product has been discontinued.



Highly purified preparation. Useful in structural and functional studies of collagen metabolism. Catalyzes hydrolysis of native collagen to peptides.

Highly purified preparation. Useful in structural and functional studies of collagen metabolism. Catalyzes hydrolysis of native collagen to peptides. Has an optimal pH of 7.0-8.0.

Warning

Toxicity: Harmful (C)

Unit Definition

One BTC (bovine tendon collagen) unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent from collagen per min at 37°C, pH 7.2.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or freeze (-20°C) for long-term storage. Stock solutions are stable for up to 4 days at 4°C and for up to 3 months at -20°C.

Reconstitute with 100 µl 50 mM Tris, 10 mM Ca(OAc)₂, pH 7.2.

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Safety Information

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