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234155 Sigma-Aldrich

Collagenase, Type II, Clostridium histolyticum

Collagenase, Type II, Clostridium histolyticum, is a crude collagenase preparation that contains higher clostripain and tryptic activity than Type I.

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
packaging   100 mg in Plastic ampoule (234155-100MG)
foreign activity   Non-specific proteolytic ≥50 fluorescence units/mg
mfr. no.   Calbiochem
specific activity activity   ≥200 collagenase units/mg dry wt
storage condition   OK to freeze
  desiccated
storage conditions   +2C to +8C
form   lyophilized
solubility   aqueous buffer: soluble
shipped in   ambient

Description

General description

Crude collagenase preparation that contains higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required.

Crude collagenase solid prepared to contain higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required. Has an optimal pH of 7.0-8.0.

Other Notes

Keller, S., and Mandl, I. 1963. Arch. Biochem. Biophys.101, 81.

Reconstitution

Unstable in solution; reconstitute just prior to use.

Unit Definition

One unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent from collagen per min at 37°C, pH 7.2.

Warning

Toxicity: Harmful (C)

Safety & Documentation

Safety Information

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