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251760 Sigma-Aldrich

DAR-4M - Calbiochem

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

Synonym: DAR-4M - Calbiochem, Diaminorhodamine-4M

  • Empirical Formula (Hill Notation) C25H26N4O3

  • Molecular Weight 430.50

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Indicators and Probes, Fluorescent Probes, Labels, Particles and Stains, Fluorescent Stains, Nitric Oxide Probes,
Quality Level   100
assay   ≥98% (HPLC)
form   solution
mfr. no.   Calbiochem®
storage condition   OK to freeze
  protect from light
storage conditions   -20C
color   red-violet
fluorescence   λex ~560  nm
  λem ~575 nm
shipped in   ambient

Description

General description

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.

Packaging

1 mg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: no

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Irritant (B)

Physical form

In DMSO.

Reconstitution

Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.

Other Notes

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Legal Information

Not available for sale in Japan.

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Safety Information

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