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  • 264150 - Caspase-3 Substrate IV, Fluorogenic - CAS 1177935-21-5 - Calbiochem

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264150 Sigma-Aldrich

Caspase-3 Substrate IV, Fluorogenic - CAS 1177935-21-5 - Calbiochem

Fluorogenic caspase-3 substrate.

Synonym: Z-DEVD-AFC

  • CAS Number 1177935-21-5

  • Empirical Formula (Hill Notation) C36H38F3N5O14

  • Molecular Weight 821.71

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Properties

Related Categories Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Protease, Substrates by Enzyme More...
packaging   1 mg in Plastic ampoule (264150-1MG)
  5 mg in Plastic ampoule (264150-5MG)
color   white to off-white
feature productdata solubility   DMSO (10 mg/ml)
fluorescence   λem ~505 nm
  λex ~400  nm
form   solid
mfr. no.   Calbiochem
shipped in   ambient
storage condition   OK to freeze
purity   ≥95% by HPLC
storage conditions   -20C

Description

Biochem/physiol Actions

Cell permeable: no

Primary Target
Fluorogenic caspase-3 substrate.

Product does not compete with ATP.

Reversible: no

General description

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.

Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Other Notes

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.

Packaging

1, 5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

Warning

Standard Handling (A)

Sequence

Z-Asp-Glu-Val-Asp-AFC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
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