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287891 Sigma-Aldrich

5,6-Dichloro-1-β-D-ribofuranosylbenzimidazole - CAS 53-85-0 - Calbiochem

A potent, ATP-competitive, and specific inhibitor of casein kinase II (IC₅₀ = 6 µM).

Synonym: 5,6-Dichlorobenzimidazole Riboside, DRB, Casein Kinase II Inhibitor VII, 5,6-Dichloro-1-β-D-ribofuranosylbenzimidazole - CAS 53-85-0 - Calbiochem

  • CAS Number 53-85-0

  • Empirical Formula (Hill Notation) C12H12Cl2N2O4

  • Molecular Weight 319.14

  •  MDL number MFCD00036785

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Properties

Related Categories A to C, Biochemicals and Reagents, Casein Kinase G, Enzyme Inhibitors, Enzyme Inhibitors by Enzyme,
Quality Level   100
assay   ≥98% (TLC)
form   solid
potency   6 μM IC50
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +15C to +30C
color   white
solubility   DMSO: 3 mg/mL
  ethanol: 3 mg/mL
shipped in   ambient
InChI   1S/C12H12Cl2N2O4/c13-5-1-7-8(2-6(5)14)16(4-15-7)12-11(19)10(18)9(3-17)20-12/h1-2,4,9-12,17-19H,3H2/t9-,10-,11-,12-/m1/s1
InChI key   XHSQDZXAVJRBMX-DDHJBXDOSA-N

Description

General description

A potent, ATP-competitive, and specific inhibitor of casein kinase II (IC50 = 6 µM). Reported to inhibit insulin-stimulated nuclear and cytosolic p70S6 kinase in CHO cells. Accentuates premature termination of transcription. Has been used to inhibit RNA polymerase II transcription which may be dependent on casein kinase II. Inhibits germinal vesicle breakdown in bovine oocytes. Induces G1/S cell cycle arrest.

Accentuates premature termination of transcription close to the promoter. Useful for isolation of RNA sequences near promoter sites that are responsible for mRNA formation. Useful for reversible superinduction of human fibroblast cultures. Potent, ATP-competivie and specific inhibitor of casein kinase II (IC50 = 6 µM). Has been used to inhibit RNA polymerase II transcription, which may be dependent on casein kinase II. Inhibits germinal vesicle breakdown in bovine oocytes.

Packaging

50 mg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: no

Primary Target
casein kinase II

Product competes with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Preparation Note

May require warming (47°C) to achieve complete dissolution.

Reconstitution

Room temperature (+20°C). Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Ethanol stock solutions are stable for up to 6 weeks at 4°C or for up to 3 months at -20°C.

Other Notes

Kim., S.J., and Kahn, C.R. 1997. Biochem. Biophys. Res. Commun. 234, 681.
Farin, C.E., and Yang, L. 1994. Mol. Reprod. Dev.37, 284.
Giardina, C., and Lis, J.T. 1993. J. Biol. Chem. 268, 23806.
Zandomeni, R., et al. 1986. J. Biol. Chem.261, 3414.
Mittleman, B., et al. 1983. J. Mol. Biol. 165, 461.
Zandomeni, R., et al. 1983. J. Mol. Biol. 167, 561.
Zandomeni, R., et al. 1982. Proc. Natl. Acad. Sci. USA79, 3167.
Stewart, M.W., et al. 1977. J. Gen. Virol.37, 221.

Safety & Documentation

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