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OmniPur CHAPS - CAS 75621-03-3 - Calbiochem

Synonym: 3-[(3-Cholamidopropyl)-dimethylammino]-propane- sulfonate, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, CHAPS

  • CAS Number 75621-03-3

  • Empirical Formula (Hill Notation) C32H58N2O7S

  • Molecular Weight 614.88

  •  MDL number MFCD00012116

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Properties

Quality Level   100
form   crystalline powder
mol wt   micellar avg mol wt 6150
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +15C to +30C
aggregation number   10
color   white
CMC   6 mM (20-25°C)
transition temp   cloud point >100 °C
shipped in   ambient
SMILES string   C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI   1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
InChI key   UMCMPZBLKLEWAF-RFCNGIAKSA-N

Description

General description

Zwitterionic detergent ideal for solubilization of membrane proteins, analytical, diagnostic, chromatography, electrophoresis work, and enzymology and immunology applications.
Suitable for Molecular Biology

Conductivity (10%, water): ≤50 µmho
DNase: None detected
Protease: None detected
Purity (TLC): Single spot
RNase: None detected
Solubility: 20% in water, passes test





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS’ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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