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309800 Sigma-Aldrich

Dihydroethidium - CAS 38483-26-0 - Calbiochem

A cell-permeable, chemically-reduced ethidium derivative.

Synonym: 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE

  • CAS Number 38483-26-0

  • Empirical Formula (Hill Notation) C21H21N3

  • Molecular Weight 315.41

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Probes, Labels, Particles and Stains, Fluorescent Stains, Nucleic Acid Stains, Nucleic Acid Stains For Microscopy More...
packaging   25 mg in Glass bottle (309800-25MG)
color   pink
form   solid
mfr. no.   Calbiochem
shipped in   ambient
solubility   DMF: soluble
  DMSO: 20 mg/mL
storage condition   OK to freeze
  protect from light
purity   ≥95% by HPLC
storage conditions   -20C

Description

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Useful fluorogenic probe for the detection of reactive oxygen species (ROS)

Product does not compete with ATP.

Reversible: no

General description

A cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry.

A cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.

Other Notes

Haugen, T.S., et al. 1999. Eur. Respir. J.14, 1100.
Mancini, M., et al. 1997. J. Cell Biol.138, 449.
Carter, W.O., et al. 1994. J. Leukoc. Biol.55, 253.
Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991. Cytometry 12, 687.
Bucana, C.D., et al. 1990. Exp. Cell Res.190, 69.
Rothe, G., and Valet, G. 1990. J. Leukoc. Biol.47, 440.

Packaging

25 mg in Glass bottle

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

B

Safety & Documentation

Safety Information

RTECS 
SF7935000

Documents

Certificate of Analysis

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Protocols & Articles
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