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3110 Sigma-Aldrich

LIGHT DIAGNOSTICS Respiratory Virus Panel I DFA, ~50 tests

  •  eCl@ss 32161000

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Properties

biological source   mouse
clone   monoclonal
mfr. no.   Chemicon®
  LIGHT DIAGNOSTICS
application(s)   immunofluorescence: suitable
conjugate   FITC conjugate
detection method   fluorescent
shipped in   wet ice

Description

General description

Diagnostics Kit

Components

DFA Reagents - (Catalog No. Adeno - 5016, Influenza A - 5017, Influenza B - 5018, Para 1 - 5019, Para 2 - 5020, Para 3 - 5021, RSV - 5022). Seven (7) x 2 mL dropper vials containing fluorescein-labeled monoclonal antibody directed against: adenovirus, influenza A, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, and RSV with protein stabilizer, Evans blue and 0.1% sodium azide (preservative).

Antigen Control Slides - (Catalog No. Adeno - 5009, Influenza A & B - 5010, Para 1,2,3 - 5011, RSV - 5012). Four (4) control slides containing wells with positive and negative control cells. Each positive control well is infected with one of the following: adenovirus, influenza A, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, and RSV. Negative control wells contain uninfected cells.

Phosphate-Buffered Saline - (Catalog No. 5087). One (1) packet of PBS salts.

Tween 20 / Sodium Azide Solution (100X) - (Catalog No. 5037). One (1) 10 mL vial containing Tween 20 / Sodium Azide concentrate.

Mounting Fluid - (Catalog No. 5013). One (1) 10 mL dropper vial containing Tris-buffered glycerin, a fluorescence enhancer and 0.1% sodium azide (preservative).

Storage and Stability

When stored at 2-8ºC, the Respiratory Panel 1 DFA Kit is stable up to the expiration date printed on the kit label. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the kit expiration date.

Warnings and Precautions:

· Sodium Azide, present in the labeled monoclonal antibodies, PBS, and Mounting Fluid is toxic is ingested. Sodium Azide can form potentially explosive metal azides with lead and copper pipes. Upon disposal, flush with large amounts of water to prevent azide build-up.

· Pooling or alteration of any reagent may cause erroneous results.

· Do not substitute reagents from other manufacturers.

· Do not allow the shell vials or slides to dry at any time during the staining procedure.

· Handle all specimens and materials coming in contact with them as potentially infectious materials. Decontaminate with 0.05% Sodium hypochlorite prior to disposal.

· Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks or flame. Avoid breathing vapor. Use adequate ventilation.

· Avoid contact with Evans blue (present in the DFA Reagents ) as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

· PBS with Tween 20/Sodium Azide should not be used in viral isolation procedures.

· Do not mouth pipette reagents.

· Do not expose reagents to bright light during storage or incubation.

· Alteration of protocol provided may cause erroneous results.

· When staining multiple samples on a slide, avoid cross contamination between samples.

Disclaimer

For in vitro Diagnostic Use

CE Mark

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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