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  • 317496 - Dual Specificity Protein Phosphatase 1/6 Inhibitor, BCI - CAS 15982-84-0 - Calbiochem

317496 Sigma-Aldrich

Dual Specificity Protein Phosphatase 1/6 Inhibitor, BCI - CAS 15982-84-0 - Calbiochem

The Dual Specificity Protein Phosphatase 1/6 Inhibitor, BCI controls the biological activity of Dual Specificity Protein Phosphatase 1/6. Primarily used for Phosphorylation & Dephosphorylation apps.

Synonym: Dual Specificity Protein Phosphatase 1/6 Inhibitor, BCI - CAS 15982-84-0 - Calbiochem, NSC 150117, (E)-2-Benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one, MAP Kinase Phosphatase 1/3 Inhibitor, BCI, Dusp1/MKP1 Inhibitor, Dusp6/MKP3 Inhibitor, PTP Inhibitor XXVI

  • CAS Number 15982-84-0

  • Empirical Formula (Hill Notation) C22H23NO

  • Molecular Weight 317.42



Related Categories Biochemicals and Reagents, Enzyme Inhibitors, Enzyme Inhibitors by Enzyme, Enzymes, Inhibitors, and Substrates, L to O,
Quality Level   100
assay   ≥95% (HPLC)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  protect from light
storage conditions   +2C to +8C
color   yellow
solubility   DMSO: 50 mg/mL
shipped in   ambient


General description

A cell-permeable cyclohexylamino-indenone compound that acts as an allosteric inhibitor against substrate binding-induced MAPK phosphatase activity of Dusp1/CL100/HVH1/MKP1/PTPN10 and Dusp6/MKP3/PYST1 (57% and 30% reduction, respectively, of 0.21 and 2.1 µg Erk2-induced OMFP dephosphorylation by 0.21 µg Dusp6) by locking Dusp1 and Dusp6 in their low-activity conformation, while exhibiting little activity toward Cdc25B, DUSP3/VHR, DUSP5, or PTP1B. Shown to effectively enhance cellular Erk phosphorylation in HeLa cells overexpressing human Dusp1 and Dusp6 (EC50 = 11.5 and 12.3 µM, respectively). FGF signaling activation by BCI treatment (5 to 10 µM) during zebrafish embryo development is reported to expand myocardial progenitors population at the expense of other progenitor cell types, resulting in enlarged heart size.


10 mg in Glass bottle

Packaged under inert gas


Toxicity: Standard Handling (A)


Following reconstiution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Molina, G., et al. 2009. Nat. Chem. Biol.5, 680.

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