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324393 Sigma-Aldrich

Anti-Dopamine D₂ Receptor Rabbit pAb

lyophilized, clone, Calbiochem®

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Properties

Related Categories Alphabetical Index, Antibodies, DM-DP, Primary Antibodies
clone   polyclonal
biological source   rabbit
species reactivity   human, rat
form   lyophilized
shipped in   ambient
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C

Description

General description

Anti-Dopamine D₂ Receptor, rabbit polyclonal, specifically recognizes the dopamine D2 receptor in rat brain. Has minimal reactivity with the D2S receptor. It is validated for use in ELISA, WB, & IHC.

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa dopamine D2 receptor protein.

Recognizes the dopamine D2 receptor in rat brain. Does not cross-react with other dopamine receptors and exhibits minimal cross-reactivity with the D2S short receptor.

Immunogen

Human

a synthetic peptide (CAARRAQELEME) corresponding to amino acids 272-282 of human D₂ dopamine receptor

Application

ELISA (see comments)

Immunoblotting (1:800)

Frozen Sections (1:5000, see application references)

Physical form

Undiluted serum.

Reconstitution

Reconstitute lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Packaging

100 μL in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Rat brain

Other Notes

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.

This antibody is highly specific for the immunizing peptide sequence and is suitable for detection of both the D2S and D2L dopamine receptors in human and rat samples. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted antiserum detects two bands at ~48 and ~51 kDa in an analysis using whole rat brain homogenates. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections, a 1:5000 dilution of the reconstituted antiserum demonstrates specific staining of rat brain sections including the plasma membrane in the cortex, caudate putamen, globus pallidus, thalamic nucleus, supra optic nucleus, and the dentate gyrus. Variables associated with assay conditions will dictate the proper working dilution.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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