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324405 Sigma-Aldrich

Anti-Dopamine D₄ Receptor Rabbit pAb

lyophilized, clone, Calbiochem®

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Properties

Related Categories Alphabetical Index, Antibodies, DM-DP, Primary Antibodies
clone   polyclonal
biological source   rabbit
species reactivity   rat, human
form   lyophilized
shipped in   ambient
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C

Description

General description

Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC.

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor.

Recognizes the ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors.

Immunogen

Human

a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor

Application

ELISA (see comments)

Immunoblotting (1:500)

Immunohistochemistry (1:10,000)

Physical form

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 μL in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Rat brain

Other Notes

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.

This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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